| 1 | Steve Lombardozzi Jr. | 2B | St. Petersburg College | 5' 11" | 195 | 23 | 13 |
| 2 | Roger Bernadina | LF | — | 6' 2" | 210 | 27 | 91 |
| 3 | Wilson Ramos | C | — | 6' 1" | 241 | 24 | 113 |
| 7 | Ivan Rodriguez | C | — | 5' 9" | 205 | 39 | 44 |
| 8 | Danny Espinosa | 2B | Long Beach State | 6' 0" | 205 | 24 | 158 |
| 10 | Corey Brown | LF | Oklahoma State | 6' 1" | 200 | 25 | 3 |
| 11 | Ryan Zimmerman | 3B | Virginia | 6' 3" | 215 | 27 | 101 |
| 12 | MSMatt Stairs | RF | — | 5' 9" | 200 | 43 | 56 |
| 13 | ACAlex Cora | SS | Miami (FL) | 6' 0" | 200 | 35 | 91 |
| 14 | Chris Marrero | 1B | — | 6' 3" | 229 | 23 | 31 |
| 15 | JJJerry Hairston Jr. | 2B | Southern Illinois | 5' 10" | 195 | 35 | 75 |
| 17 | Sean Burnett | P | — | 5' 11" | 185 | 29 | 69 |
| 19 | LNLaynce Nix | CF | — | 6' 1" | 220 | 30 | 124 |
| 20 | Ian Desmond | SS | — | 6' 3" | 217 | 26 | 154 |
| 21 | Jason Marquis | P | — | 6' 1" | 220 | 33 | 25 |
| 22 | Drew Storen | P | Stanford | 6' 1" | 200 | 24 | 73 |
| 23 | Collin Balester | P | — | 6' 4" | 190 | 25 | 23 |
| 24 | RARick Ankiel | CF | — | 6' 1" | 210 | 32 | 122 |
| 25 | Adam LaRoche | 1B | Seminole State College (OK) | 6' 2" | 205 | 31 | 43 |
| 26 | Jesus Flores | C | — | 6' 1" | 210 | 26 | 30 |
| 27 | Jordan Zimmermann | P | Wisconsin - Stevens Point | 6' 2" | 225 | 25 | 29 |
| 28 | Jayson Werth | RF | — | 6' 5" | 235 | 32 | 150 |
| 29 | Yunesky Maya | P | — | 6' 0" | 215 | 30 | 10 |
| 30 | Jonny Gomes | LF | Santa Rosa Junior College | 6' 1" | 230 | 30 | 43 |
| 31 | John Lannan | P | Siena | 6' 4" | 235 | 27 | 33 |
| 32 | Tom Gorzelanny | P | Triton College | 6' 3" | 218 | 29 | 30 |
| 35 | Craig Stammen | P | Dayton | 6' 2" | 228 | 27 | 7 |
| 36 | Tyler Clippard | P | — | 6' 3" | 200 | 26 | 72 |
| 37 | Stephen Strasburg | P | San Diego State | 6' 5" | 239 | 23 | 5 |
| 38 | Michael Morse | LF | — | 6' 5" | 245 | 29 | 146 |
| 40 | Chien-Ming Wang | P | Taipei Physical Education College | 6' 4" | 225 | 31 | 11 |
| 41 | Brad Peacock | P | Palm Beach State CC | 6' 1" | 207 | 23 | 3 |
| 43 | BBBrian Bixler | SS | Eastern Michigan | 6' 1" | 195 | 28 | 79 |
| 45 | DSDoug Slaten | P | Los Angeles Pierce College | 6' 5" | 215 | 31 | 31 |
| 46 | Tommy Milone | P | Southern California | 6' 0" | 215 | 24 | 5 |
| 48 | Ross Detwiler | P | Missouri State | 6' 5" | 210 | 25 | 15 |
| 52 | Ryan Mattheus | P | Sacramento City College | 6' 3" | 220 | 27 | 35 |
| 55 | Brian Broderick | P | Grand Canyon | 6' 6" | 205 | 25 | 11 |
| 57 | Chad Gaudin | P | — | 5' 10" | 190 | 28 | 10 |
| 59 | Atahualpa Severino | P | — | 5' 11" | 220 | 26 | 6 |
| 60 | TCTodd Coffey | P | — | 6' 4" | 240 | 31 | 69 |
| 61 | Livan Hernandez | P | — | 6' 2" | 245 | 36 | 31 |
| 63 | Henry Alberto Rodriguez | P | — | 6' 1" | 225 | 24 | 59 |
| 65 | CKCole Kimball | P | Centenary College of New Jersey | 6' 3" | 240 | 26 | 12 |